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You can now design molecular beacons for NASBA® assays for single template or multiplex reactions.
Scorpions®
Beacon Designer™ supports the design of Scorpions® primers and probes.
Designing MethyLight assays
Beacon Designer™ designs TaqMan® probes & methyl sensitive PCR primers. The MethyLight assays involves treatment of genomic DNA with sodium bisulphite followed by an alkaline treatment. Beacon Designer™ displays the corresponding bisulphite treated strand when the genomic sequence is provided. The program designs primers and TaqMan® probes for the specified CpG islands. You can also design suitable control primers and probes by designing them for unmethylated and untreated DNA sequences. These primers and TaqMan® probes are highly specific as they are designed by avoiding regions of significant cross homology identified by automatically interpreting BLAST search results. Template structures are also avoided during the design.
Specific and Efficient Design: How Beacon Designer™ Makes it Possible?
You can BLAST search sequences and search for template structures from within the program. The results of both these searches are used while designing primers and probes. The regions that exhibit significant cross homologies and template structures are avoided automatically during the design.
"Using Beacon Designer, we designed qPCR primers which performed extremely well in our experiments. I found the program both powerful and user-friendly." -Mr. José Nuno Leitao, Institute for Molecular and Cell Biology, IBMC, Portugal.
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