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"From several software packages that I have tested Beacon Designer™ is probably the best." -Dr. Ivan Bendezu, Letterkenny Institute of Technology, Ireland.
Salient Features Supported for MethyLight Assays in Beacon Designer™
a) Prediction of CpG islands in the DNA sequence.
b) Design methyl specific primers and TaqMan® probes.
c) Design suitable control primers and TaqMan® probes for corresponding unmethylated and untreated sequences.
d) Analyze pre-designed/published primers and/or TaqMan® probes and design of compatible oligos.
e) Primers and TaqMan® probes are designed by avoiding regions of homology identified by automatically interpreting BLAST search results.
Multiplex Real Time PCR Assays
With Beacon Designer™ you can design TaqMan® based multiplex real time PCR assays for upto five sequences including reference gene selection. Read more
"I successfully designed different sets of Taqman probes and primers for my genes of interest using Beacon Designer. The multiplex capability of the software was especially useful for designing my assays." -Ms. Sandra Meryl Rodrigues, North Carolina State University, US.
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