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"I designed PCR primers using Primer Premier and I found it to be extremely robust, efficient and user friendly software." -Dr. Irfan Durrani, Agricultural University Peshawar, Pakistan.
Razieh Fatehi, Sharifeh Khosravi, Maryam Abedi, Rasoul Salehi, Yousof Gheisari.
Journal of Research in Medical Sciences. 2017; Sep 26;22:102. doi: 10.4103/jrms.JRMS_136_17.
"Word of mouth has it that the Beacon Designer™ software our group uses gives the best results. All the groups are now flocking to our lab to use our copy. What a nuisance! I wish they would spring for their own copy" -Shelley Zhang, Univ. of Louisville, Baxter Bl
Primer extension may be hindered due to the presence of template structures at extension temperature. To avoid all such regions, where the template may fold upon itself, the program utilizes a proprietary algorithm to check for possible secondary structures within the template at a folding temperature you specify. The regions involved in the formation of a secondary structure are underlined in the sequence view and are avoided while designing primers.
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